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Tasso Inc
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Jackson Laboratory
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Huachuang Securities Co Ltd
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Jichuan Pharmaceuticals Group Co Ltd
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Shanghai Model Organisms Center
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Taconic Biosciences
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Huachuang Securities Co Ltd
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Jackson Laboratory
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Huachuang Securities Co Ltd
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Journal: Drug Testing and Analysis
Article Title: Improvement of EPO Transgene Detection From Polymeric Dried Blood Spots for Antidoping Application
doi: 10.1002/dta.70008
Figure Lengend Snippet: Validation of RM‐EPO detection from TASSO DBS. A. Selectivity, sensitivity (Limit of detection) and reproducibility of detection are shown. PCR was performed using Taqman EPO assay on DNA extracted from DBS TASSO M‐20 loaded with 10 different blood samples spiked at 0–1500–2500–5000 c/mL RM‐EPO. Cq values are indicated. Examples of size of amplified products are shown for bloods spiked at 5000 c/mL. Reproducibility experiments were performed after DNA extraction from the second and third spot of the TASSO device. B. CP performed on the fourth spot of TASSO M‐20 (“B analysis”). PCR was performed using NMI CP on DNA extracted from the fourth spot (last spot) of DBS TASSO M‐20 loaded with blood samples spiked at 5000 c/mL RM‐EPO. Cq values are indicated. Examples of size of amplified products are shown.
Article Snippet: The best results were achieved with polymeric DBS, and a full validation was performed for the detection of the EPO transgene using
Techniques: Biomarker Discovery, Amplification, DNA Extraction